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. 2011 May 16;193(4):769–784. doi: 10.1083/jcb.201008050

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© 2011 Chen et al.

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Figure 5. — Sarm1 knockdown influences spine morphology and density in mature neurons. (A) Dendritic spine morphology. At 13 DIV, cultured hippocampal neurons were transfected with Sarm1i¹ or pSuper vector control. 4 d later, cells were fixed and stained with GFP antibodies. A region indicated by the arrows in the whole cell image is shown enlarged in on the bottom. Bars: (top) 30 µm; (bottom) 5 µm. (B) Quantification of the spine density, the width of the spine head, and the length of spines. Error bars indicate mean values ± SEM. ***, P < 0.0005.