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. 2011 May 16;193(4):785–798. doi: 10.1083/jcb.201010099

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© 2011 da Silva Lopes et al.

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Figure 4. — Myofibril structure is maintained during FRAP. (A) We followed fluorescence recovery in areas of 3 × 3 or 8 × 8 sarcomere lengths to distinguish movement of titin-eGFP from myofibril rearrangement. Bar, 10 µm. (B) Comparison of fluorescence recovery for 1 × 1 (control), 3 × 3, or 8 × 8 sarcomere lengths. As the region of interest increases in size recovery takes longer, emphasized in the center of the photobleached area (A, recovery at 8 × 8). (C) The mobile fractions were independent from the size of the bleached area. The recovery was homogeneous and sarcomere structure maintained over the region of interest (control, n = 4; 3 × 3, n = 4; 8 × 8, n = 3). s.l., sarcomere length. Error bars indicate SEM.