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. 2011 May 16;193(4):785–798. doi: 10.1083/jcb.201010099

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© 2011 da Silva Lopes et al.

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Figure 7. — Titin-eGFP dynamics in cardiomyocytes is independent from contractility. (A) Cardiomyocytes were treated with the myosin inhibitor 2,3-butanedione monoxime (BDM), maintained in a high calcium concentration (HCa, 2.8 mM), or treated with a combination of BDM and 2.8 mM calcium. Selected regions from nonbeating cells expressing titin-eGFP were photobleached. Bar, 10 µm. (B and C) The fluorescence recovery was monitored over time. The mobility of titin-eGFP of nonbeating cells was equally efficient compared with beating control cells (control, n = 4; BDM, n = 3). (D and E) FRAP analysis with BDM treatment was repeated in cells cultured in medium with high calcium levels. The decreased dynamics of titin-eGFP could not be rescued by inhibition of contraction (control, n = 4; BDM + HCa, n = 3). *, P < 0.05. Error bars indicate SEM.