Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 16;193(4):655–665. doi: 10.1083/jcb.201011038

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Vega et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — RhoA inhibits Rac1-driven protrusive activity. (A) F-actin distribution in PC3 cells after transfection with siRNAs. Boxed regions are shown at higher magnification on the right. Bars, 20 µm. (B) FLIM images from PC3 cells cotransfected with the Rac1 biosensor and indicated siRNAs. Higher Rac1 activity is shown in “warmer” colors. Bars, 10 µm. (C, top) Kymographs of boxed regions in B: protruding membrane region (1) and control nonprotruding region (2); 1 frame/min (20 min), t = time. (C, bottom) Graph of threshold relative Rac1 activity values in protruding and control regions; **, P < 0.01. (D) Mean total Rac1 biosensor lifetime of cells transfected with the indicated siRNAs. Error bars indicate ± SEM.