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. 2000 Jun 15;14(12):1471–1484.

Figure 5.

Figure 5

Expression of FK mRNA in Arabidopsis embryo development. Sections were hybridized with RNA probes containing the entire FK coding sequence. Wild-type embryos hybridized to antisense probe: (A) early globular stage; (B) late globular stage; (C) heart stage; (D) early torpedo stage; (E) torpedo parasagittal section; (F,G) torpedo stages; (H) bent-cotyledon stage; (I) mature embryo [arrows indicate weak hybridization signals in the shoot meristem (top arrow) and root tip (bottom arrow)]. (J) Wild-type torpedo stage embryo hybridized to FK sense probe as a negative control; (K,L) fk-X224 mutant embryos hybridized to antisense probe. Expression is absent; (K) fk heart stage; (L) fk torpedo stage; (M–O) wild-type torpedo stage embryo transverse serial sections of a transition zone in the hypocotyl region hybridized to the antisense probe. FK expression is stronger in the first (M), more basal section and becomes weaker in the following sections (N,O). Scale bars, 20 μm (A = B, C = D = K, M = N = O); 40 μm (E = F = G = H, I = J).