Table 1.
Mass spectral analysis of sterols from fk mutants shows accumulation of Δ8,14 sterols
| Sterol
|
M+
|
Seedlings
|
Callus Cells
|
|||
|---|---|---|---|---|---|---|
| WT
|
fk-X224
|
WT
|
fk-X224
|
fk-UU2099
|
||
| Cholesterol | 368* | 8.6 | 16.1 | 0.5 | 2.5 | 1.1 |
| 24-Methylcholesta-5,22-dien-3β-ol | 380* | 2.0 | − | − | − | − |
| 24-Methylenecholesterol | 380* | (+) | − | − | − | − |
| Campesterol | 382* | 13.1 | (+) | 16.2 | (+) | − |
| Campestanol | 444 | (+) | − | 0.5 | − | − |
| Stigmasterol | 394* | 8.9 | 10.5 | 0.5 | 0.8 | 0.8 |
| Sitosterol | 396* | 67.4 | 51.9 | 59.8 | (+) | 4.2 |
| Sitostanol/Isofucosterol | 458/456 | (+) | − | 1.3 | − | − |
| Cycloartenol | 468 | − | − | 2.3 | 9.1 | 6.6 |
| 24-Methylenecycloartanol | 482 | − | − | 18.6 | 30.4 | 11.6 |
| Sterol A | 440 | − | (+) | − | 3.6 | 6.4 |
| Sterol B | 454 | − | 6.8 | − | 39.8 | 59.0 |
| Sterol C1 | 452 | − | (+) | − | 0.6 | 0.5 |
| Sterol C2 | 452 | − | 8.5 | − | 5.3 | 4.8 |
| Sterol C3 | 452 | − | 6.1 | − | 3.9 | 1.7 |
| Sterol D | 466 | − | − | − | 4.0 | 3.3 |
| Total sterols A-D | − | 21.4 | − | 57.2 | 75.7 | |
Percentages of the relative sterol composition are shown. (*) (M-HOAc)+; (−) not present; (+) traces. (WT) Wild type; fk-X224 and fk-UU2099 alleles are indicated; Sterols are listed according to mass (M); Sterols A, B, C1, C2, C3, and D are Δ8,14 sterols that were found in fk. A = 5α-Ergosta-8,14-dien-3β-ol; B = 5α-Stigmasta-8,14-dien-3β-ol; C1 = 5α-Stigmasta-8,14,Z-24(28)-trien-3β-ol; C2 = unknown Stigmastatrien-3β-ol; C3 = unknown Stigmastatrien-3β-ol; D = 4α-Methyl-5α-stigmasta-8,14,Z-24(28)-trien-3β-ol.