Table 1.
PCR amplification primers.
| Target gene | Primer sequences | Ta (°C) |
|---|---|---|
| FKBP5 | Fwd 5'-CCCTCGAATGCAACTCTCTT-3' Rev 5'-TCTCCTTTCCGTTTGGTTCT-3' |
62 |
| GAPDH | Fwd 5'-TGCACCACCAACTGCTTAG-3' Rev 5'-GATGCAGATGATGATGTTC-3' |
57 |
| IL-12p35 | Fwd 5'-CATGCTGGCAGTTATTGA-3' Rev 5'-AAGTATGCAGAGCTTGATTTT-3' |
57 |
| IL-12p40 | Fwd 5'-AGGAGAGTCTGCCCATTGAGG-3' Rev 5'-GACCTCCACCTGCCGAGAA-3' |
65 |
| IL-23p19 | Fwd 5'-CGTCTCCTTCTCCGCTTCA-3' Rev 5'-GTGCCTGGGGTGGTAGATTT-3' |
65 |
| MKP-1 | Fwd 5'-AGCAGAGGCGAAGCATCATC-3' Rev 5'-CCCAGCCTCTGCCGAAC-3' |
61 |
a) Fwd: forward primer.
b) Rev: reverse primer.
c) Ta: annealing temperature.
d) GAPDH: internal reference gene.