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. 2011 Aug 24;12:112. doi: 10.1186/1471-2350-12-112

Figure 1.

Figure 1

Gene polymorphisms were determined by DNA fragment migration profile on agarose gel and visualized by ethidium bromide staining: A. ACE I/D genotypes: D or I alleles were identified by the presence of 190 or 490 bp fragments B. NOD1 796G>A genotypes: wild-type DNA is visible as a double-band 209 bp and 170 bp (GG); the mutated DNA is visible as a single 379 bp band (AA); heterozygotes give three bands (GA); C. TLR4 3725G>C genotypes: wild-type is visible as a double-band 184 and 397 bp (GG), mutated DNA is visible as 256 and 397 bp bands (CC); heterozygotes give three bands 184, 256, and 397 bp bands (GC). Lane L is a 100 bp molecular weight marker (HyperLadder IV, Bioline GmbH, Berlin, Germany).