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. 2011 Aug 9;12:32. doi: 10.1186/1471-2121-12-32

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Copyright ©2011 Zeng et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Adipogenic and osteogenic differentiation of hMSCs. Adipogenesis was detected by the formation of intracytoplasmic lipid droplets stained with oil red O (A, no induction; B, induced cells; 100×). Osteogenic differentiation was demonstrated by calcium deposition as evidenced von Kossa staining (E, no induction; F, induced cells; 100×). Atomic force microscopy of umbilical cord-derived hMSCs upon adipogenic (C, D) and osteogenic (G, H) differentiation after 21 days in normoxic conditions were shown as error signal images (C,G) and 3-dimension graphs (D,H).