Table 2.

Overview of experiments and microscopic methods

Microscopy of infection	Detached in vitro assay	Intact in vivo assay
Cultivar/strain combinations (Figure no.)	Nandu/WT (1) Nandu/TRI5prom::GFP (2) Nandu/ΔTRI5-GFP (4) Sumai 3/TRI5prom::GFP (2*)	Nandu/TRI5prom::GFP (3*) Amaretto/TRI5prom::GFP (3*)
Time points of MZFLIII screening	all cultivar/strain combinations 6 hpi - 14 dpi (1 A, 2 A-C, 4 A-C, 2 A-C*, 3 A-F*)	6 hpi - 7 dpi (3 A-F*)
Microscopy with Axio Imager Z1	all cultivar/strain combinations (2* D)	Nandu/TRI5prom::GFP Amaretto/TRI5prom::GFP (3 G*)
CLSM	Nandu/TRI5prom::GFP (2 D, 1*)	-
SEM	Nandu/TRI5prom::GFP (2 E-F) Nandu/ΔTRI5-GFP (4 D-F)	-

The different cultivar/strain combinations, investigated time of infection and the microscopic methods used in vitro and in vivo bioassays are detailed. Respective figure numbers are given in brackets (Additional File*).