Table 4.
Nucleotide changes leading to amino acid substitutions in F, L, M2-1 and M2-2 proteins exclusively in primary clinical HRSVA strains as compared to reference strains
| Protein | Nucleotide change in Primary strains | Amino acid substitution - Position | Primary strains having substitution |
|---|---|---|---|
| F | CTC/CTT → TTT | L-15-F | RSV-1, RSV-2,RSV-7 to RSV-10 |
| L | GAC → AAC/TAC | D-148-N/Y | RSV-1, RSV-2, RSV-4 to |
| GTC/GAC → GGC | V/D-174-G | RSV-10, RSV-14 | |
| RSV-1 to RSV-9, RSV-14 | |||
| M2-1 | ATA → CTA | I-87-L | RSV-12 to RSV-14 |
| AAA → AGT | N-117-S | RSV-8, RSV-10, RSV-11 | |
| M2-2* | ATG → ACG | M-1-T | RSV-1, RSV-2, RSV-5, RSV-8 to RSV-14 |
| ACT → AAT | T-18-N | RSV-1, RSV-2, RSV-5, RSV-7 to RSV-14 | |
| AGA → AAT/AGT | R-25-N/S | RSV-1, RSV-2, RSV-5, RSV-8 to RSV-14 | |
| TTC → ATC | F-39-I | RSV-12 to RSV-14 | |
| CCA → CAA | P-44-Q | RSV-1, RSV-2, RSV-4, RSV-5, RSV-8 to RSV-14 | |
| ATG → ATA/ACG | M-48-I/T | RSV-1, RSV-2, RSV-5, RSV-7 to RSV-14 | |
| CCA → CAG | P-51-Q | RSV-12 to RSV-14 | |
| ACA → CCA | T-54-P | RSV-1, RSV-2, RSV-4 to RSV-14 | |
| ACA → GCA | T-68-A | RSV-1 to RSV-14 | |
| ATT → ACT | I-69-T | RSV-3 to RSV-10, RSV-14 | |
| ATT → ACT | I-79-T | RSV-1 to RSV-6, RSV-8, RSV-9, RSV-12 to RSV-14 | |
| GAG → GAT | E-80-D | RSV-1 to RSV-14 | |
*In the deduced M2-2 protein sequence, the RSS strain had difference at only I-48-T.