Figure 7.

 Binding of Hairy and Hairy^En to Gro deletion mutants. A diagram of the different Gro derivatives analyzed is shown. These derivatives were assayed for binding to Hairy, Hairy^1–286 (a truncation similar to Hairy^En but lacking En^R), and Hairy^En. Equivalent aliquots of labeled Gro derivatives are shown as a control for the strength of the interactions (Input lanes). Gro^ΔWD and Gro^WD show weak or no binding to either Hairy or Hairy^En. Gro derivatives with progressive WD repeat deletions also show very weak binding to Hairy, but Gro^Δ6 and Gro^Δ5,6 interact significantly with Hairy^En.