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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biophys Chem. 2011 May 27;159(1):120–128. doi: 10.1016/j.bpc.2011.05.014

Figure 2.

Figure 2

Sedimentation velocity analysis of dp8 binding to PKR. A) Normalized g(s*) distributions obtained at a fixed concentration of PKR (16 μM) with the indicated concentration of of dp8: 0 μM (black), 8 μM (red), 16 μM (blue) and 32 μM (green). The distributions are normalized by peak maximum. Conditions: rotor speed, 50,000 RPM; temperature, 20°C; inteference optics; scan interval, one minute. Note that the free dp8 is not resolved in these distributions because the the scan range was chosen to emphasize the higher-s features and diffusional broadening reduces resolution of the low-s region in the time derivative method. Inset: bdp8 binding to PKR analyzed by fluorescence-detected sedimentation velocity. c(s) distributions of 250 nM bdp8 (black) and 250 nM bdp8 + 10 eq. PKR (red). Conditions: rotor speed, 50,000 RPM; temperature, 20°C; fluorescence optics.