Figure 5.

Dissociation of the SecA dimer by signal peptide. A) Plot of apparent dimer dissociation constants (K_d,app) vs. signal peptide concentration. SecA dimer dissociation constants were obtained at 200 mM KCl by sedimentation velocity measurements using fluorescence optics. The solid line is a fit of the data to equation 3 performed as described in the text. The best-fit parameters are: β₁₁ = 0.158 (μM) ⁻¹ and β₂₂ = 0.00264 (μM) ⁻². This figure was adapted from reference [64].