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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: J Mol Biol. 2011 Jul 29;412(3):354–364. doi: 10.1016/j.jmb.2011.07.055

Figure 4. Trap blocks strand transfer in the presence of E478Q mutant RT.

Figure 4

(a) A representative 10 % denaturing gel of strand transfer reactions in the absence (a) and presence (b) of NC. Substrates used are as described in Figure 1b. Lanes 1–8 represent independent reactions with reagents added as shown above the gels by the (+) or (−) signs. TP represents transfer product, DE, donor extension product while P is unextended labeled primer. The numbers on the side indicate the expected lengths of these products in nucleotides. Panel (c) summarizes the effects of the blocking polymer on donor extension and transfer efficiency. Black bars represent products without (−) NC and gray bars are products with (+) NC. Products analyzed here; PD, representing measured donor extension efficiency without trap; PDT, measured donor extension efficiency in the presence of trap; PDA(D), measured donor extension efficiency in the presence of unprimed acceptor; PDA(TE), measured transfer efficiency without trap; PDAT(D), measured donor extension efficiency in the presence of unprimed acceptor and trap; PDAT(TE), measured transfer efficiency in the presence of trap; PA, measured extension efficiency on a primed acceptor, and PAT, measured extension efficiency on primed acceptor with trap.