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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: J Mol Biol. 2011 Jul 29;412(3):354–364. doi: 10.1016/j.jmb.2011.07.055

Figure 5. Trap blocks strand transfer with AZT resistant-RT (AZTr-RT).

Figure 5

Experiments examining the behavior of a mutant reverse transcriptase with higher primer-template affinity. Panel (a) displays a gel image showing products of transfer reactions performed using AZTr-RT in the absence of NC, while panel (b) shows products of reactions performed in the presence of NC. The conditions of transfer reactions were as described under Materials and Methods. The numbers 1–8 shown above the gel images represent different experiment and control reactions. The (+) and (−) shown on top indicates the specific additives for each reaction. The labels P, DE and TP denote labeled unextended primer, donor extension and transfer product, respectively. The numbers 38, 40 and 50 represent the size of primer, donor extension and transfer product, respectively in nucleotides.

Panel (c) is a bar graph summarizing donor and transfer efficiency of independent reactions calculated and averaged from at least three reactions. Error bars represent standard error of the mean. Other abbreviations used are as described under Figure 4.