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. 1997 Nov 15;11(22):2952–2957. doi: 10.1101/gad.11.22.2952

Figure 2.

Figure 2

 Maternal gro is required for the ventral repression directed by the minimal zen VRR. Wild-type nuclear cycle 13 embryos (A,B) or gro nuclear cycle 11 (C) or nuclear cycle 13 (D) embryos containing transgenic P-element constructs (right), which are essentially identical to those used previously (Jiang et al. 1993). Lateral views of syncytial blastoderm embryos are shown. lacZ mRNA was detected by whole mount in situ hybridization. The construct in A contains the minimal eve-stripe 2 enhancer (MSE) driving lacZ expression (MSE–lacZ); the constructs in B–D contain two copies of the minimal 180-bp zen VRR upstream of the MSE (VRR–MSE). Black boxes represent the AT-rich corepression elements; shaded ovals represent Dorsal-binding sites. The arrow in C shows the VRR-directed expression of lacZ across the entire ventral surface.