Figure 3.

 Analysis of the partial DPE sequence, G^A/_TCG, in a TATA-less and a TATA-containing promoter. (A) A DPE-related motif in the TATA-less Drosophila Abd-B core promoter is important for transcriptional activity. Wild-type and DPE mutant versions of the Abd-B core promoter (comprising sequences from −17 to +41 relative to the major upstream start site) were constructed, as depicted at the bottom. These templates were transcribed in vitro with the Drosophila SK nuclear extract, and the resulting transcripts were subjected to primer extension analysis. Transcriptional activity is reported as relative to that of the wild-type Abd-B minimal promoter. (B) The DPE-like sequence in the TATA-containing Drosophila hsp70 promoter does not compensate for the loss of the TATA box. Wild-type and mutant versions of the TATA-containing hsp70 core promoter (comprising sequences from −35 to +40 relative to the RNA start site) were constructed, as shown. Transcription reactions were performed with the indicated template DNAs and Drosophila SK nuclear extract. Transcriptional activity is reported as relative to that of the wild-type hsp70 minimal promoter.