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. 1997 Nov 15;11(22):3020–3031. doi: 10.1101/gad.11.22.3020

Figure 4.

Figure 4

Figure 4

 Mutation of the DPE sequence does not affect transcript stability. (A) In vitro transcription reactions were performed with the hb P2–Antp P2 (TATA+Inr+DPE+) and hb P2–Antp P2*DPE (TATA+Inr+DPE−) hybrid minimal promoters (Burke and Kadonaga 1996). After 30-min reaction time, α-amanitin was added (to 4 μg/ml final concentration) to inhibit transcription, and the reaction mixtures were further incubated for the indicated times to determine the stability of the transcripts with the wild-type DPE relative to those with the mutant DPE. The reverse transcription products are indicated by the bracket (right). Note that the preferential use of the +1 start site in the DPE-containing promoter is probably due to the optimal positioning of the DPE to the +1 start site relative to the +5 site, as discussed in Burke and Kadonaga (1996). (B) Graph of data shown in A.