Skip to main content
. Author manuscript; available in PMC: 2012 Sep 9.
Published in final edited form as: Vaccine. 2011 Jan 14;29(39):6755–6762. doi: 10.1016/j.vaccine.2010.12.064

Figure 4. DNA vaccination using pLCMV-NP with EP is completely protective against lethal challenge.

Figure 4

Mice (n=6 per group) were immunized three times i.m. using EP with either 35 μg of empty vector control plasmid (pVAX) or 35 μg of pLCMV-NP, with two weeks between injections. Positive control mice were injected i.p. with 2×105 PFU of LCMV. Eight weeks following infection or the final injection of pDNA, mice were challenged i.c. with 20LD50 LCMV and animal survival is displayed in the graph. Mice were observed for 16 days following challenge and results from LCMV immune, pLCMV-NP vaccinated, and pVAX vaccinated (dotted line) are shown. Experiments were performed at least three times in independent experiments and data are representative of the results.