Table 1.

Putative classification of feline leukemia virus (FeLV) infections in Florida pumas (Puma concolor coryi) based on enzyme-linked immunosorbent assay (ELISA) antibody, polymerase chain reaction (PCR), ELISA antigen, and immunofluorescence assay (IFA) results and clinical findings 1990 to April 2007.

Classification
Puma no.	Date	Gender	Age (yr)	Event	ELISA antibody	PCRa	ELISA antigen	IFA	Viral culture	FeLV- related disease	FIVb status
Regressive Infection
FP11	8 January 1998	♀	16	Capture	(Pc) 0.251	NDd	Ne,f	ND	ND	No	N
FP12	4 January 1993	♂	12	Capture	(P) 0.28	N	Nf	ND	ND	No	ND
FP36	8 January 1992	♀	5.5	Capture (P)	0.251	N	Nf	ND	ND	No	P
	22 February 1994		7.5	Capture	(N) 0.249g	ND	Nf	ND	ND	No	P
FP65	20 November  1997	♂	1	Capture	(P) 0.362	N	Nf	ND	ND	No	N
	8 March 1999		3.4	Capture	(N) 0	ND	Nf	ND	ND	No	E
FP67	22 April 2002	♀	4.8	Capture	(P) 0.26	N	N	ND	ND	No	N
FP69	3 January 2005	♀	7.8	Capture	(P) 0.252	N	N	ND	ND	No	P
FP78	14 December  2001	♀	4	Capture	(P) 0.454	N	N	ND	ND	No	P
FP82	6 December 2002	♀	6	Capture	(P) 0.262	N	N	ND	ND	No	N
FP99	26 January 2001	♂	0.9	Capture	(P) 0.296	N	N	ND	ND	No	E
	6 November 2001		1.7	Capture	(P) 0.426	ND	N	ND	ND	No	P
FP104	2 April 2001	♂	0.6	Capture	(P) 0.292	N	N	ND	ND	No	N
	13 December  2002		2.1	Capture	(N) 0.132	ND	N	ND	ND	No	P
FP107	1 November 2001	♀	1.6	Capture	(P) 0.324	N	N	ND	ND	No	P
	6 December 2004		4.7	Capture	(N) 0.230	ND	N	ND	ND	No	P
FP108	3 November 2001	♂	0.9	Capture	(P) 0.273	N	N	ND	ND	No	P
FP140	14 November  2005	♀	3.5	Capture	(P) 0.298	N	N	ND	ND	No	P
FP141	30 November  2005	♂	3.5	Capture	(P) 0.297	ND	N	ND	ND	No	P
FP143	9 January 2006	♂	1.5	Capture	(P) 0.467	ND	N	ND	ND	No	N
FP145	16 February 2006	♀	1.5	Capture	(P) 0.347	ND	N	ND	ND	No	P
FP146	27 February 2006	♂	3	Capture	(P) 0.267	ND	N	ND	ND	No	P
UCFP43	29 August 2001	♂	2.5	Capture	(P) 0.277	N	N	ND	ND	No	N
Latent infection
FP96	1 November 2001	♂	1.6	Capture	(P) 0.337	P	N	ND	ND	No	P
	29 May 2002		1.8	Necropsy	ND	P	N	ND	ND	No	ND
FP100	31 January 2001	♂	4	Capture	(P) 0.3	P	N	ND	ND	No	N
	6 January 2004		7	Capture	(N) 0.155	P	N	ND	ND	No	N
FP110	13 February 2002	♀	1.1	Capture	(P) 0.256	N	N	ND	ND	No	N
	25 November  2002		2	Capture	N	P	N	ND	ND	No	N
FP118	5 March 2003	♀	0.9	Capture	N	P	N	ND	ND	No	N
	5 April 2003		1	Necropsy	ND	ND	N	ND	ND	No	ND
FP119	2 April 2003	♂	1	Capture	N	P	N	ND	ND	No	P
	17 November  2004		2.6	Capture	N	N	N	ND	ND	N/D	ND
Persistent infection
FP115	26 November  2002	♀	4.5	Capture	(P) 0.499	P	P	Inconclu- sive	P	No	P
	18 May 2003		5	Necropsy	ND	P	P	P	P	Yes	ND
FP122	30 January 2004	♀	2.3	Capture	N	P	P	P	P	Yes	N
	14 February 2004		2.4	Necropsy	ND	P	P	ND	P	Yes	ND
FP123	2 February 2004	♂	3.5	Capture	N	P	P	P	P	No	P
	15 March 2004		4	Necropsy	ND	P	P	ND	ND	Unknown	ND
FP132	17 March 2004	♂	3	Capture	N	N	N	ND	ND	No	N
	22 July 2004		3.3	Necropsy	ND	P	P	P	ND	Yes	ND
Transient viremia or persistent infection
FP109	10 February 2002	♂	10	Capture	N	ND	N	ND	ND	No	N
	24 January 2003		11	Capture	(P) 0.546	P	P	Inconclu- sive	P	Yes	N
	26 February 2003		11.1	Necropsy	N/D	ND	Unknown	ND	N/D	Unknown	ND

^aData from Brown et al. (2008).

^bFIV = feline immunodeficiency virus.

^cP = positive.

^dND = not determined.

^eN = negative.

^fFlorida Fish and Wildlife Conservation Commission (unpubl. data).

^gOptical density continued to decline to 0.179 by last sampling March 1997.