Table 2.

Tilt and roll angles between Tg and adjacent bases

Sequence context	Inter-base pair	Tilt (°)	Roll (°)	Inter-base pair	Tilt (°)	Roll (°)
5′-G-Tg-G	G-Tg	14.9	−16.2	Tg-G	−9.1	−3.5
5′-A-Tg-G	A-Tg	60.4	−44.7	Tg-G	−11.0	12.7
5′-C-Tg-G	C-Tg	−46.1	11.8	Tg-G	9.6	−6.7
5′-T-Tg-G	T-Tg	−45.6	−6.5	Tg-G	15.0	1.3
B-DNA		4.5	−2		4.5	−2

Tilt and roll angles were calculated with Curves.³¹ In the 5′-A-Tg-G sequence context, Tg adopts an orientation that ismore extrahelical than in other complexes, which explains the marked difference in the angle values. The tilt and roll angles for B-DNAwere calculated and averaged from the structure of a B-form DNA dodecamer (PDB ID code: 1BNA).³²