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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: J Immunol. 2011 May 11;186(12):6771–6778. doi: 10.4049/jimmunol.1100099

FIGURE 1.

FIGURE 1

IFN-γ downregulates β-catenin signaling pathway. PFA (A) or U251MG astroglioma cells (C) were left untreated or treated with IFN-γ (100 ng/ml) for 24 h prior to transfection with TOPflash luciferase and Renilla luciferase constructs. After resting for 4 h, the cells were cultured with or without initial treatment of IFN-γ. Dual luciferase activity was measured 24 h later. Data shown are normalized to Renilla activity. Background level of dual luciferase reading is between 0.35–0.13 and is indicated in first two columns of A and C. PFA (B) or U251MG cells (D) were treated with or without IFN-γ for 48 h (B) or for 1, 24, or 48 h (D), and expression of hypophosphorylated/active β-catenin level was measured by conventional intracellular flow cytometry. E, PDA or U87MG cells were transfected with TOPflash or FOPflash with Renilla. Firefly luciferase activity over Renilla activity was measured 24 h posttransfection. Data represent a minimum of three experiments. *p < 0.05.