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. 1998 Apr 15;12(8):1134–1144. doi: 10.1101/gad.12.8.1134

Figure 1.

Figure 1

 RecQ helicase, in conjunction with RecA and SSB proteins, initiates homologous pairing. Labeled, linear pUC19 DNA and pUC1950 scDNA were incubated with: (Lane 1) RecA and SSB proteins; (lane 2) RecQ helicase and SSB protein; (lanes 3–8) all three proteins; (lane 9) RecQ helicase and RecA protein alone. Slower migrating species that represent joint molecules, labeled Joint Molecules, are observed only in the presence of all three proteins. In a control reaction, RecA and SSB proteins were incubated with labeled linear pUC19 ssDNA, cold linear pUC19 dsDNA, and pUC1950 scDNA to produce bona fide joint molecules (lane 10). All reactions were carried out as in Materials and Methods. Bands corresponding to linear ssDNA and dsDNA are also indicated to the left.