Table 2.
Oligonucleotides used to construct DNA substrates
| 1a | 5′- | G | A | C | G | C | T | G | C | C | G | A | A | T | C | C | T | A | C | C | A | G | T | G | C | C | T | T | G | C | T | A | G | G | A | C | A | T | C | T | T | T | G | C | C | C | A | C | C | T | G | C | A | G | G | T | T | C | A | C | C | C | -3′ | |||
| 2a | 5′- | T | G | G | G | T | G | A | A | C | C | T | G | C | A | G | G | T | G | G | G | C | A | A | A | G | A | T | G | T | C | C | T | A | G | C | A | A | T | G | T | A | A | T | C | G | T | C | A | A | G | C | T | T | T | A | T | G | C | C | G | T | T | -3′ | ||
| 3a | 5′- | C | A | A | C | G | G | C | A | T | A | A | A | G | C | T | T | G | A | C | G | A | T | T | A | C | A | T | T | G | C | T | A | G | G | A | C | A | T | G | C | T | G | T | C | T | A | G | A | G | G | A | T | C | C | G | A | C | T | A | T | C | G | A | -3′ | |
| 4a | 5′- | A | T | C | G | A | T | A | G | T | C | G | G | A | T | C | C | T | C | T | A | G | A | C | A | G | C | A | T | G | T | C | C | T | A | G | C | A | A | G | G | C | A | C | T | G | G | T | A | G | A | A | T | T | C | G | G | C | A | G | C | G | T | -3′ | ||
| 5 | 5′- | A | C | A | G | C | A | C | C | A | G | A | T | T | C | A | G | C | A | A | T | T | A | A | G | C | T | C | T | A | A | G | C | C | A | T | C | C | G | C | A | A | A | A | A | T | G | A | C | C | T | C | T | T | A | T | C | A | A | A | A | G | G | A | -3′ | |
| 6 | 5′- | T | C | C | T | T | T | T | G | A | T | A | A | G | A | G | G | T | C | A | T | T | T | T | T | G | C | G | G | A | T | G | G | C | T | T | A | G | A | G | C | T | T | A | A | T | T | G | C | T | G | A | A | T | C | T | G | G | T | G | G | C | T | G | T | -3′ |
| 7 | 5′- | T | C | C | T | T | T | T | G | A | T | A | A | G | A | G | G | T | C | A | T | T | T | T | T | G | C | G | G | A | T | G | G | C | T | T | A | G | A | G | C | T | T | A | A | T | T | G | C | -3′ | ||||||||||||||||
| 8 | 5′- | G | C | A | A | T | T | A | A | G | C | T | C | T | A | A | G | C | C | A | T | C | C | G | C | A | A | A | A | A | T | G | A | C | C | T | C | T | T | A | T | C | A | A | A | A | G | G | A | -3′ |
a
These sequences are the same as those for oligonucleotides 1–4 described in Benson and West (1994).