Table 2.
Experimental investigation of the metastatic potential of Nf2-deficient tumor cells by tail vein injection
| Tumor cell linea
|
Tumor type
|
Recipient
|
No. of cells injected
|
Length of experimentb
|
No. of lung metastases
|
|---|---|---|---|---|---|
| NfFB1 | fibrosarcoma | F1 | 1 × 106 | 21 | >200c |
| nude | 2 × 106 | 24 | >200c | ||
| NfFB2 | fibrosarcoma | F1 | 2 × 106 | 12 | >200c |
| F1 | 2 × 106 | 13 | >200c | ||
| NfFB3 | fibrosarcoma | F1 | 2 × 106 | 17 | >200c |
| p53FB1 | fibrosarcoma | F1 | 1 × 106 | 21 | 0 |
| F1 | 2 × 106 | 21 | 0 | ||
| p53FB2 | fibrosarcoma | F1 | 2 × 106 | 21 | 0 |
| F1 | 2 × 106 | 21 | 0 | ||
| p53FB3 | fibrosarcoma | F1 | 1 × 106 | 21 | ∼10 |
| nude | 2 × 106 | 25 | ∼10 | ||
| F1 | 1 × 106 | 21 | >100d | ||
| p53FB4 | fibrosarcoma | F1 | 1 × 106 | 16 | >200c |
| F1 | 2 × 106 | 17 | >200c | ||
| NfOS1 | osteosarcoma | F1 | 1 × 106 | 21 | ∼20 |
| F1 | 1 × 106 | 31 | ∼50 | ||
| nude | 2 × 106 | 24 | >200c | ||
| NfOS2 | osteosarcoma | F1 | 2 × 106 | 26 | ∼5 |
| F1 | 1.4 × 106 | 21 | ∼5 | ||
| p53OS1 | osteosarcoma | F1 | 2 × 106 | 21 | 0 |
| F1 | 2 × 106 | 21 | 0 | ||
| F1 | 0.5 × 106 | 21 | 0 | ||
| F1 | 2 × 106 | 21 | 1 | ||
| p53OS2 | osteosarcoma | F1 | 1 × 106 | 21 | 0 |
| F1 | 1 × 106 | 31 | 0 |
a
Nf and p53 designations indicate Nf2 and p53 deficiency, respectively.
b
In days.
c
The animal was moribund and was sacrificed before the endpoint of the experiment, apparently because of a large metastatic load.
d
Shortly after injection of the tumor cells, we discovered that this mouse was pregnant; pups were delivered 2 weeks postinjection. Pregnancy may have had a positive effect on the growth of tumor cells in this mouse.