Table 1.
Anchorage-independent growth of CEF-expressing c-Jun and v-Jun bZip mutants
| Culture
|
Number of cells seededa
|
Plating efficiency (%)
|
||
|---|---|---|---|---|
| 1 × 104
|
2 × 103
|
1 × 103
|
||
| Uninfected | − | − | — | |
| R vector | − | − | — | |
| R–c-Jun wild type | + | 444/442 | 222/168 | 21 |
| R–c-Jun-m0 | + | 198/258 | 142/108 | 13 |
| R–c-Jun-m1 | 92/86* | 26/23# | − | <1 |
| R–v-Jun | + | 550/496 | 142/164 | 19.5 |
| R–v-Jun–m0 | + | 210/178 | 102/88 | 9.6 |
| R–v-Jun–m1 | 24/32* | 10/14* | 4/8* | <1 |
| R–Fra2 | +** | 52/32** | 14/16** | 1.8 |
| R–ATF2 | − | − | − | |
| RD–v-Jun–m1 + R vector | − | 16/12* | N.D. | |
| RD–v-Jun–m1 + R–v-Jun–m0 | N.D. | 64/44 | N.D. | |
| RD–v-Jun–m0 + R vector | N.D. | 194/220 | N.D. | |
| RD–v-Jun–m0 + R–v-Jun–ml | N.D. | 398/346 | N.D. | |
Colony formation in agar of CEF cultures fully infected with the retroviruses indicated. Duplicates were seeded on 60-mm plates at the densities indicated, and colonies were scored after 2 weeks.
a
Plating efficiency: percentage of single cells of 1 × 103 developing into colonies. (+) Numerous colonies; (−) no colonies present; (*) many colonies show necroses; (**) microcolonies; (N.D.) not determined.