Table 2.
Bacterial strains, phage, and plasmids
|
|
Relevant genotype
|
Source
|
|---|---|---|
| Strains | ||
| MG1655 | F− | S. Adhya |
| CH1105 | MG1655 ΔlacZM15 | S. Adhya |
| CH1200 | CH1105 Δcya854 | S. Adhya |
| MG0002 | CH1105 Δ(manA–uidA) 1298 zdg-232::Tn10 | this study |
| MG0003 | CH1105 zdg-232::Tn10 | this study |
| DM0007 | MG0002 ΔgalE::cat | this study |
| DM0008 | CH1200 Δ(manA–uidA)1298 zdg-232::Tn10 | this study |
| DM0009 | DM0008 ΔgalE::cat | this study |
| DM0011 | DM0009 [λ B3001] | this study |
| DM0012 | DM0009 [λ B3002] | this study |
| DM0013 | DM0009 [λ B3003] | this study |
| DM0018 | DM0009 [λP1galE–lacZΔ(galT)galK] | this study |
| DM0021 | DM0009 P1galE–lacZΔ(galT)galK | this study |
| DM0022 | DM0021 [λ B3001] | this study |
| DM0026 | DM0022 ΔgalR::cat | this study |
| JT7 | galR+[λ ΔgalR::cat] | S. Adhya |
| JT30 | ΔgalR::kan | S. Adhya |
| JT32 | ΔgalR(A–B)::kan | S. Adhya |
| DM0081a | DM0022 ΔgalR(A–B)::kan | this study |
| DM0081b | DM0081agalR–D258N | this study |
| DM0082 | DM0081 agalR–R325H | this study |
| DM0083 | DM0081agalR–E230K | this study |
| DM0108 | DM0081agalR–N259M | this study |
| DM0109 | DM0081agalR–T322R | this study |
| DM0110 | DM0081agalR–H327R | this study |
| Phage | ||
| Y392 | λc1857 gal+int− | this study |
| Y3001 | λP1+P2-galE–lacZ(galT)galK | this study |
| B3000 | λ imm21 galK+Δatt P′ P+ | this study |
| B3001 | B3000 P2+P1−galE–gusA bla | this study |
| B3002 | B3000 P2−P1+galE–gusA bla | this study |
| B3003 | B3000P2+P1+galE–gusA bla | this study |
| W30 | λcI− | NIH collection |
| W248 | λcI−h80 | NIH collection |
| B10 | λimm21 cI− | NIH collection |
| B 482 | λimm21 cI−h80 | NIH collection |
| Plasmids | ||
| pAP1 | pPROEX galR+ | this study |
| pI24 | pBR322Δ(EcoRI–PvuII) gal+ | S. Adhya |
| pRAJ275 | gusA+ | this study |
| pAA121(p14A) | P2+P1−galEK | S. Busby |
| pAA121(p19T) | P2−P1+galEK | S. Busby |
| pSA805 | pI24Δ(EcoRI–BstEII) galP2+P1− | this study |
| pSA806 | pI24Δ(EcoRI–BstEII) galP2−P1+ | this study |
| pSA809 | pSA805(HindIII–BspEI)gusA+ | this study |
| pSA808 | pSA806(BstEII–BspEI)lacZ+ | this study |
| pTS8 | P1−P2−gal ETK P−lacZ λattPOP′int+bla | this study |
| pSA814 | pTS8 Δ (PstI–BspEI) P2+P1−galE–gusA | this study |
| pMT4 | pBR322 Δ (PstI–EcoRI) galR(A–B)::kan | J. Tokeson |