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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Cancer Lett. 2011 May 10;308(1):23–32. doi: 10.1016/j.canlet.2011.04.010

Fig.4.

Fig.4

Anchorage-independent growth of cells expressing TGFBI. TGFBI- and empty vector-transfected (A) H522 and (B) MCF-7 cells were cultured in soft agar and colony formation was scored three weeks later. The cells transfected with TGFBI formed more than 5-fold fewer colonies in soft agar than those transfected with vector controls. Graphs (left panel) are mean±SD of colony forming units (CFU) per 1,000 cells from three independent experiments. *, p<0.05 versus vector controls. Images of colonies that formed in soft agar are represented on the right panel (magnification 40×). Arrows indicate colonies with >30 cells.