Table 5. Morphometric data of lymphoid and myeloid cell subsets in ileum of pigs immunized with either vaccine candidate F4ac+non-ETEC strain or with combination of levamisole and F4ac+non-ETEC. The results are expressed as the mean values and standard deviations of the number of cells per µm2 of tissue section field; in every sample the cells were counted in 12 randomly chosen fields with the average area of 672387.5 µm2.
| Treatment Of pigsa | Mean±SD(×10−5 ) number of lymphoid and myeloid cells residing ileal mucosa | ||||
|---|---|---|---|---|---|
| CD3 | CD45RA | CD45RC | CD21 | SWC3 | |
| Noneb | 3.14±0.47 | 12.2±4.17 | 2.98±0.7 | 4.93±1.67 | 1.42±0.86 |
| F4ac+ non ETEC | 21.6±9.47* | 113.0±21.8*** | 15.2±9.97 | 84.2±16.8*** | 9.26±1.25*** |
| Levamisole +F4ac+ non-ETEC | 18.6±3.39*** | 155.0±65.3* | 19.5±6.85* | 88.4±23.5** | 10.6±3.13** |
a
Groups comprised five 4-weeks-old pigs each.
b
Control pigs received saline as a placebo. Significantly different at:
*
P<0.05,
**
<0,01 or
***
<0.001 than in the control nontreated pigs.