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. 2010 Mar 20;54(1):e6. doi: 10.4081/ejh.2010.e6

Figure 3.

Figure 3

(A) Immunoblot expression of RANK-ligand in cocultured cells. RANK-ligand production results more expressed at T/4 respect to days T/0–T/2. (3B–B1) Transmission electron microphotographies of immature osteoclast membrane showing the colocalization between RANKl clathrin where RANKl is immunogold labelled with particles of 15 nm (black arrows) and clathrin clusters are labelled with 10 nm of gold particles (white arrows). (C) Double labeling clathrin /actin expression on immature osteoclast developed by coculture system. Scale bar: 3A–B1 1 µm 3C: 50 µm.