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. 2010 Jun 14;54(2):e16. doi: 10.4081/ejh.2010.e16

Figure 4.

Figure 4

Immunohistochemical localization of MSTN in sea bass larvae. All panels are counter-stained with haematoxylin. A, C, E: diploid animals; B, D, F: triploid animals. A–B Sagittal sections of 2-day larvae. In both diploids (A) and triploids (B), a moderate immunostaining is found in the epithelium of developing intestine (I), in skeletal muscle (M) and in the epithelium of skin (arrow). Spinal cord (SC) is negative. C–D) Sections of 6-day larvae of both diploids (C) and triploids (D), showing a moderate immunoreactivity in skeletal muscle (M), intestinal epithelium (I), liver (L) and skin (arrows). E–F Sagittal sections of 10-day larvae of both diploids (E) and triploids (F), showing immunostaining in intestinal epithelium (I) and pancreas (P). Cartilage (c) is negative. L: liver. Bars (A) 20 µm, (B) 20 µm, (C) 40 µm, (D) 20 µm, (E) 20 µm, (F) 20 µm.