Table 3. Immunohistochemical localization of IGF-II in diploids (D) and triploids (T) of sea bass.
Tissue | 2 dph | 6 dph | 10 dph | 30–45 dph | 60 dph | 74 dph | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
D | T | D | T | D | T | D | T | D | T | D | T | |
Gill epithelium | * | * | - | - | - | - | + | +/- | + | + | + | +/- |
Heart | * | * | - | - | - | - | + | +/- | +/- | +/- | +/- | +/- |
Gut epithelium | + | +/- | + | +/- | ++ | ++ | + | + | +/- | +/- | +/- | +/- |
Liver | * | * | + | +/- | +/- | +/- | + | +/- | + | +/- | + | +/- |
Kidney | * | * | +/- | - | +/- | - | - | - | - | - | - | - |
Pancreas | * | * | - | - | - | - | - | - | - | - | - | - |
Skin | + | + | + | + | + | + | + | + | + | + | + | + |
Skeletal muscle | + | + | + | + | + | +/- | +/- | +/- | +/- | +/- | +/- | +/- |
Yolk sac | +/- | +/- |
Staining:
not detectable;
slight but above background levels;
moderate;
marked staining.
Tissue not found on the sections examined at this stage.