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. 2010 Jun 14;54(2):e23. doi: 10.4081/ejh.2010.e23

Figure 1.

Figure 1

TLC of gangliosides extracted under various conditions. (A) Effect of temperature on dry acetone extractability of GM1 ganglioside. (1) direct extraction with C-M-W; (2) extract with dry acetone at −20°C; (3): C-M-W extract after previous extraction with dry acetone at −20°C; (4): acetone extract at room temperature; (5): C-M-W extract after previous extraction with dry acetone at room temperature. Note: GM1 ganglioside detected with ChTB (GD1b ganglioside band was also detected with much lower sensitivity). GM1 content in all acetone extracts was below the detection level of very sensitive cholera toxin staining. (B) Comparison of GM1 extraction with dry acetone and with aqueous acetone. (6) Direct extraction with C-M-W; (7) extract with dry cold acetone at −20°C. (8): C-M-W extract after previous extraction with dry acetone at −20°C; (9) extracts with mixture acetone-water 9:1 shows significant loss of GM1; (10) C-M-W extracts after previous extraction with acetone-water mixture.