Figure 6.

Ezrin in the cytosolic fraction (A) and in the whole cellular extracts (B) of colon adenocarcinoma cell variants and normal NRK cells. Bars represent the mean from three independent experiments each performed in triplicate (± SD). Results are expressed as units of integrated optical density (IOD). The statistical significance of the difference was confirmed by Student's t-test, P<0.05; *indicates values significantly different from those for NRK cells; #indicates values significantly different for human adenocarcinoma sublines in comparison to parental LS180 cells. β-tubulin was used as the loading control during all Western blotting experiments.