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. 1999 May 15;13(10):1234–1239. doi: 10.1101/gad.13.10.1234

Figure 3.

Figure 3

Characterization of RNAP II’s effects on splicing. Splicing reactions with β-globin pre-mRNA were reconstituted with S100 extract plus 250 ng of SR proteins and the indicated additional components. (a) Extra SR proteins do not affect splicing. Reaction mixtures were supplemented with 120 ng of RNAP IIA (lane 2), RNAP IIO (lane 3), or 120 ng of additional HeLa SR proteins (lane 4). (b) The CTD is necessary but not sufficient. Reactions mixtures were supplemented with 100 ng of RNAP IIA (lanes 2,10), 100 ng of RNAP IIO (lanes 3,11), 60 ng of GST (lanes 4,15), 30 or 60 ng of GST–CTD (lanes 5,6), 30 or 60 ng of phosphorylated GST–CTD (lanes 7,8), or 30, 60, or 120 ng of RNAP IIB (lanes 12–14). (c) Time course of splicing. Reaction mixtures were supplemented with 100 ng of RNAP IIO (lanes 5–8) and incubated at 30°C for the indicated times.