Figure 2.
Muscle biopsies immediately fixed after removal from the body. a, b. Samples fixed with 2.5% glutaraldehyde and 2% paraformaldehyde, post-fixed with 1% OsO4 and embedded in Epon. The cytoplasm shows well-preserved sarcomeres; glycogen (G) mostly occurs as clusters. Mitochondria (arrows) show numerous cristae and electron dense matrix granules, and frequently surround the lipid droplets (L). c. Samples fixed with 2% paraformaldehyde and 0.5% glutaraldehyde, and embedded in LR White. The sarcomeres are well visible and the mitochondria, which are lined between them, show still recognizable cristae (inset). Bars: a, c 1 µm; b, inset 0.5 µm.