Figure 4.

Expression of TrkA mRNA in normal and castrated rats. Real-time RT-PCR was performed on vas deferens, vesicular gland and dorsal and ventral prostate. The data were expressed as the mean±standard error. Significant differences in TrkA mRNA levels between the calibrator sample (normal rats) vs castrated rats were determined by using Student’s t-test for independent samples. The level of statistical significance was set at p<0.05 for all. * = p<0.05; # p=0.10. Note that TrkA mRNA levels increase in the vesicular gland and in the dorsal and ventral prostate of the castrated rats. These results were consistent with the immunohistochemical staining results. In the vas deferens the difference was not statistically significant.