Figure 4.

Immunostaining of RyRs in cochleae of the PND 14 group. (A) ×100. Negative control. (B) ×100. Cochleae were structurally fully developed. The strongest expression of RyRs was found in Corti’s organ and second strongest was in SGNs. In stria vascularis, low expression was found in basilar cells. (C) ×400. The differentiation of Corti’s organ was close to that of an adult. RyRs were widely expressed in IHCs and concentrated in the region under the nuclei. Strong expression of RyRs was found in the region under the nuclei of OHCs and supporting cells, including Deiters’ cells, Hensen cells, Claudius’ cells, pillar cells, and inner sulcus cells. (D) ×200. The intercellular space of SGNs became large. Significant expression of RyRs was found in the plasma.