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. 2011 Sep;8(9):1482–1491. doi: 10.1016/j.hrthm.2011.03.061

Figure 6.

Figure 6

Multiparametric optical mapping of rat left ventricle (original signal traces, unfiltered, obtained from tissue within 4 × 4 pixel square outlined in top panel). Em1–4 correspond to emitted fluorescence during exposure to excitation sources Ex1–4, respectively. A: In the presence of the [Ca2+]i dye fura-2 only, no significant fluorescence signal is detected when illuminated by sources 1 and 2 (excitation sources for di-4-ANBDQPQ). B: Simultaneous ratiometric Vm and ratiometric [Ca2+]i optical mapping after additional loading with di-4-ANBDQPQ. EM1/EM2 and EM3/EM4 show normalized Vm and CaT signals after calculation of corresponding source signal ratios (EM1/EM2 signals in A are normalized with respect to corresponding signal amplitudes in B). Scale bar = 5 mm. Note up to 10-fold difference in Y-axis scaling for Em3 and Em4, compared to EM1 and Em2.