Figure 4.

her5 misexpression does not affect intermediate endodermal derivatives. Embryos were injected into a dorsolateral (a–c) or lateral (d–f) blastomere at the 16-cell stage with 4.5 pg of her5 + 5 pg of GFP mRNAs, and are observed at 72 hr. (a,b,d,e) Bright field and fluorescence whole-mount views, anterior to the left. (c,f) Cross-sections at the levels indicated, with GFP protein revealed by immunocytochemistry (brown). The progeny of the injected blastomere (arrows) contributed normally to the pharyngeal pouches (a–c) and gut derivatives (d–f). (f) Cell labeling in the swim bladder (area surrounded by filled dots; open dots line the intestine proper).