Fig. 3.

MGF-Ct24E mainly enhanced the cell proliferation via the MAPK-Erk1/2 pathway. Immunoblotting analysis demonstrated that MGF-Ct24E treatment induced a significant enhancement of phosphorylated Erk1/2 expression (a) but had a blur effect on Akt compared to the vehicle control (b). Cells were incubated with MGF-Ct24E containing inhibitor of the MAPK-Erk1/2 or PI3K pathway (a and b), and the addition of PD or LY to MGF-Ct24E supplemented cultures reduced cell proliferation by 70% or 4%, respectively (c). ^# P < 0.01 versus C, *P < 0.05 versus MGF-Ct24E. C: The vehicle control containing α-MEM and 0.1% DMSO, PD: PD98059, LY: LY294002, E: MGF-Ct24E, n > 3