Figure 1.

Cloning and characterization of SNIP1. (A) Putative genomic structure of human SNIP1 gene is shown. Black boxes represent untranslated regions and the white boxes represent translated regions. The exon numbers are noted inside their respective white boxes with the sizes of introns written at the bottom. Numbers at the edges of the boxes denote the amino acid number from the human protein sequence. (B) Schematic diagram of human SNIP1 protein is shown with two conserved domains noted in shaded boxes, which are a bipartite NLS and a FHA. Numbers at the edges of the boxes denote the amino acid number from the deduced protein sequence. The sequence of the human SNIP1 protein is shown with the arrow indicating the starting methionine and an asterisk star representing the stop codon. The NLS is enclosed in a box and the FHA domain is underlined. (C) Commercially available Northern blot was purchased from Clontech and hybridized with the carboxy-terminal fragment of SNIP1. The sizes of three transcripts are indicated. (D) COS-1 cells were transfected with HA-tagged SNIP1 constructs as indicated. The specificity of an antibody against SNIP1 was monitored by Western blotting of the lysates. HA–SNIP1 and HA–SNIP1-C are indicated by arrows. (NS) Nonspecific band. (E) Western blot of cell lysates from various cell lysates. A single band of ∼50 kD was detected. (F) Western blot of lysates from various adult mouse tissues.