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. 2000 Jul 1;14(13):1605–1616.

Figure 5.

Figure 5

Injection of SNIP1 into Xenopus embryos leads to a suppression of Gsc expression and anterior truncations. A total of 1 ng of SNIP1-FL (C,D,H), or SNIP1-N (E,F,I) or 2 ng of SNIP1-C (B,G) RNA were injected into the two dorsal blastomeres of a 4-cell embryo. Embryos were allowed to develop until stage 10.5 and fixed for whole mount in situ analysis for Xgsc expression, or embryos were cultured until stage 33, fixed, and photographed. SNIP1-FL or SNIP1-N-injected embryos exhibited a suppression of Xgsc expression as compared with the uninjected control (A) or to the SNIP1-C injected embryos. At stage 33, SNIP1-C injected embryos were phenotypically normal, whereas SNIP1-FL and SNIP1-N-injected embryos often showed loss of anterior structures.