Figure 3.

 SEL-10 is a member of the CDC4 family of F-box/WD40-repeat proteins. The CDC4 sequence (accession no. X05625) is from GenBank. There are other potential CDC4 family members in the database; limited sequence data for a potential SEL-10 ortholog is given in part D. For further discussion of the CDC4 family, see Kumar and Paietta (1995) and Bai et al. (1996). Reverse contrast letters indicate amino acid identity. (A) Schematic depiction of SEL-10 and CDC4, drawn to scale. The percentage of identical amino acids in each region is indicated. Members of the CDC4 family all have this general organization, with some variability in the length and sequence of their amino and carboxyl termini. (B) Alignment of SEL-10 and CDC4 F-Boxes. (C) Alignment of WD40 repeats from SEL-10 and CDC4. (D) Alignment of SEL-10 and a potential human ortholog. Partial sequence of a human cDNA encoding a sequence highly similar to the carboxyl terminus of SEL-10 was obtained from Genbank (accession no. H22962) and was extended into the WD40 repeats by direct sequencing of clone ym50h08.s1 (G. Wu and J. Kitajewski, unpubl.). The predicted amino acid sequence encoded by the available human cDNA sequence is shown. The database also contains partial sequence information for a rat cDNA (Genbank accession no. H34371) that is predicted to encode a peptide that is 100% identical to the last 35 amino acids of the available human sequence (data not shown).