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. 1997 Dec 1;11(23):3182–3193. doi: 10.1101/gad.11.23.3182

Figure 4.

Figure 4

 Coimmunoprecipitation of C. elegans SEL-10 with either C. elegans LIN-12(intra) or murine Notch4(int3) from transfected 293T cells. (A) Samples were immunoprecipitated with anti-HA antibody and the Western blot was probed with anti-myc to visualize SEL-10myc (top) or anti-HA to visualize LIN-12(intra)HA (bottom). Arrow indicates the expected mobility of SEL-10myc. (B) Samples were immunoprecipitated with anti-myc antibody and the Western blot was probed with anti-HA (top) or anti-myc (bottom). Arrow indicates the expected mobility of LIN-12(intra)HA. (C) Samples were immunoprecipitated with anti-Notch4 antibody and the Western blot was probed with anti-HA to visualize SEL-10HA (top) or anti-Notch4 to visualize Notch4(int3) (bottom). Arrow indicates the expected mobility of SEL-10HA. (D) Samples were immunoprecipitated with anti-HA antibody and the Western blot was probed with anti-Notch4 (top) or anti-HA (bottom). Arrow indicates the expected mobility of Notch4(int3). For details, see Materials and Methods. (Lane 1) pQNClacZ; (lane2) pQNClin-12(intra)HA + pQNClacZ; (lane 3) pQNClacZ + pQNCsel-10myc; (lane 4) pQNClin-12(intra)HA + pQNCsel-10myc; (lane 5) mock transfected cells; (lane 6) pLNCint3 + pQNCX; (lane 7) pLNCX + pQNCsel-10HA; (lane 8) pLNCint3 + pQNCsel-10HA.

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