Table 1.

sel-10 gene dosage analysis

Relevant genotype	%Egl (n)	%Muv (n)	%Ste/Let (n)
20°
lin-12(d)/+a	6 (93)	0 (93)	0 (93)
lin-12(d)/+;sel-10b	91 (54)	0 (54)	0 (54)
lin-12(d)/+;sel-10/Dfc	92 (39)	15 (39)	0 (39)
15°
lin-12(d); +d	86 (60)	0 (60)	0 (60)
lin-12(d);sel-10/+e	98 (62)	0 (62)	0 (62)
lin-12(d);+/Dff	89 (57)i	62 (74)	10 (63)
lin-12(d);sel-10g	100 (70)i	78 (197)	55 (126)
lin-12(d);sel-10/Dfh	–	85 (34)	100 (34)j

Complete genotypes are as follows: 

^alin-12(n379)/unc-36(e251); lon-3(e2175)/him-5(e1490) 

^blin-12(n379)/unc-36(e251);lon-3(e2175) sel-10(ar41) 

^clin-12(n379)/unc-36(e251);lon-3(e2175) sel-10(ar41)/nDf42 

^dlin-12(n379); lon-3(e2175)/him-5(e1490) 

^elin-12(n379); lon-3(e2175) sel-10(ar41)/him-5(e1490) 

^flin-12(n379); lon-3(e2175)/nDf42 

^glin-12(n379); lon-3(e2175) sel-10(ar41) 

^hlin-12(n379); lon-3(e2175) sel-10(ar41)/nDf42 

Complete broods were scored by picking individual L4 animals and inspecting the plates at 24 and 48 hr for the absence of eggs on the plate (Egl) and for the presence of three or more pseudovulvae along the ventral hypodermis (Muv). Plates were then inspected after an additional three days for the presence of live progeny [“Ste/Let” refers to absence of live progeny and was, in this case, a combination of sterility (Ste) and embryonic lethality (Let)]. In some cases, broods were scored in batch for the Muv phenotype. 

ⁱPercent of fertile animals displaying the Egl defect. 

^jInferred genotype: Complete broods from lin-12(n379)/unc-36(e251);lon-3(e2175) sel-10(ar41)/nDf42) were scored. The percentage of sterile non-Unc, non-Lon progeny (34/97 = 35%) is approximately equal to that expected for lin-12(n379);lon-3(e2175) sel-10(ar41)/nDf42 genotypic class. Of the remaining 63 animals, 61/63 were unambiguously scored as heterozygotes in the next generation, whereas the remaining 2/63 did not have a sufficient number of progeny to score unambiguously.