Table 3.
Cell autonomy of sel-10 function
| A. Enhancement of lin-12(intra)a | ||
|---|---|---|
| Relevant genotype
|
% Egl (n)
|
% Mig (n)
|
| sel-10(+);dr arEx[lin-12(intra)] | 16 (88)b | 10 (57) |
|
sel-10(ar41);dr arEx[lin-12(intra)]
|
46 (136)b
|
59 (90)
|
| B. Cell ablationc | ||
| Relevant genotype | % 0AC (n) | |
| unoperated | operated | |
| lin-12(n379)/+; sel-10(+) | 10 (57) | 9 (11) |
| lin-12(n379)/+; sel-10(ar41) | 97 (71) | 83 (12) |
All strains also contained him-5(e1490). dr arEx[lin-12(intra)] = arEx152 (K. Fitzgerald, pers. comm.) is an extrachromosomal array formed by microinjection (Fire 1986; Mello et al. 1991) of pRF4 [plasmid containing rol-6(su1006) sequence that confers a Rol phenotype onto worms carrying the array] at 100 μg/ml and pLC8 (Struhl et al. 1993).
We infer that these Egl hermaphrodites lacked an AC because we scored additional hermaphrodites of relevant genotype sel-10; arEx[lin-12(intra)] in the L3 stage for the presence or absence of an AC and as adults for their egg-laying ability, and found that nine hermaphrodites that clearly had a single AC were non-Egl, whereas nine hermaphrodites that clearly lacked an AC were Egl.
Complete genotype: dpy-17(e164)lin-12(n379)/unc-32(e189); lon-3(e2175) sel-10(+ or ar41). “Operated” refers to worms in which Z4 was laser ablated in the early L1 stage (when the gonad primordium consisted of four cells, Z1–Z4). Worms were then scored in the L3 stage for the presence or absence of an AC.