Fig. 4.

Attenuated PERK-eIF2α signaling in rats with chronic leptin treatment. (A) Immunoblotting for phosphorylated [pPERK(Thr980)] and total PERK, phosphorylated [pEiF-2α(Ser51)] and total eIF-2α, BiP, and tubulin in adipose, liver, muscle, and pancreas tissue. (B) Results were quantified in densitometric units and expressed relative to the total protein of interest or relative to tubulin for BiP. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with control by Student's t test. (n = 6 per group.) Values are expressed as mean ± SEM.