Increased accumulation of SNC1 protein levels in cul1-7 mutant. (A) Morphology of WT, snc1, cul1-7, and cul1-7 carrying the partially complementing transgene CUL1-FLAG (CUL1-FLAG in cul1-7) (17). The picture was taken with 5-wk-old soil-grown plants. (B–D) qRT-PCR analysis of the expression of PR1 (B), PR2 (C), and SNC1 (D) in the indicated genotypes. (E) Western blot analysis of SNC1/snc1 protein levels in snc1-r1 (a loss-of-function deletion allele of SNC1), WT, snc1, cul1-7, and CUL1-FLAG in cul1-7.